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CDPK Activation in PRR Signaling

Heike Seybold1, Marie Boudsocq2, Tina Romeis1

  • 1Dahlem Centre of Plant Sciences, Plant Biochemistry, Freie Universität Berlin, Königin-Luise-Straße 12-16, 14195, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2017
PubMed
Summary

Calcium-dependent protein kinases (CDPKs) rapidly activate upon pathogen detection. An in-gel kinase assay effectively monitors transient CDPK activation and conformational changes, aiding in understanding plant immune responses.

Keywords:
CDPKElectrophoretic band shiftIn-gel kinase assayPhosphorylation targetProtoplastSignal transductionTransient expression

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