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Extending calibration-free force measurements to optically-trapped rod-shaped samples.

Frederic Català1,2, Ferran Marsà2,3, Mario Montes-Usategui1,2

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This study introduces a calibration-free method using holographic optical tweezers to measure forces on rod-shaped biological samples. This technique accurately detects drag forces, aiding microscale biological research.

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Area of Science:

  • Biophysics
  • Optical Tweezers
  • Microscale Force Measurement

Background:

  • Optical trapping is vital for microscale biological research, enabling non-invasive force measurements.
  • Accurate force quantification in optical traps requires precise calibration, challenging for diverse sample geometries.
  • Elongated biological specimens (e.g., bacteria, chromosomes) often necessitate multiple traps, complicating force determination.

Purpose of the Study:

  • To develop a calibration-free method for accurate force measurement in optical traps.
  • To address the challenge of measuring forces on elongated, rod-shaped biological samples.

Main Methods:

  • Utilized holographic optical tweezers to manipulate glass microcylinders.
  • Employed calibration-free direct detection of beam momentum for force measurement.
  • Compared experimental results with slender-body hydrodynamic theoretical calculations.

Main Results:

  • Demonstrated accurate measurement of drag forces on microcylinders without trap calibration.
  • Achieved excellent agreement between experimental data and hydrodynamic theory.
  • Validated a novel force-sensing approach for rod-shaped specimens.

Conclusions:

  • The developed method offers a reliable way to measure forces on elongated biological samples.
  • This technique has significant potential for advancing quantitative studies in microscale biophysics.
  • Calibration-free force sensing using beam momentum is a promising tool for biological research.