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Updated: Nov 3, 2025

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Positioning Accuracy in Holographic Optical Traps.

Frederic Català-Castro1,2, Estela Martín-Badosa1,2

  • 1Optical Trapping Lab, Grup de Biofotònica (BiOPT), Departament de Física Aplicada, Universitat de Barcelona, 08028 Barcelona, Spain.

Micromachines
|June 2, 2021
PubMed
Summary

Spatial light modulators (SLMs) used in optical tweezers can introduce positioning errors up to 2 nm due to their digital structure. A new correction strategy reduces these errors to 0.3 nm, improving trap accuracy.

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Area of Science:

  • Optical physics
  • Nanotechnology
  • Biophysics

Background:

  • Spatial light modulators (SLMs) are crucial for dynamic control of optical traps.
  • Holographic optical tweezers are often assumed to offer sub-nanometer positioning accuracy.
  • The impact of SLM digital features on trap positioning accuracy is not well understood.

Purpose of the Study:

  • To analyze the effects of SLM digitalization on optical trap positioning accuracy.
  • To quantify positioning errors caused by SLM look-up-tables and phase quantization.
  • To develop and validate a correction strategy for improving positioning accuracy.

Main Methods:

  • Tracking optically trapped microspheres with sub-nanometer accuracy.
  • Investigating positioning deviations related to SLM look-up-table power dependence.
Keywords:
holographic optical tweezerslaser trappingspatial light modulators

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  • Analyzing errors introduced by phase quantization in linear phase gratings.

    • Main Results:

    • SLM look-up-tables cause positioning deviations up to 2 nm at the micron scale.
    • Phase quantization introduces local errors in steering angle.
    • A developed correction strategy reduced positioning errors to 0.3 nm.

    Conclusions:

    • SLM digital features significantly impact optical trap positioning accuracy.
    • Understanding and correcting these errors is vital for high-precision optical trapping.
    • The implemented correction strategy offers a viable solution for enhancing nanometer-scale positioning control.