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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Probing Cellular Molecules with PolyA-Based Engineered Aptamer Nanobeacon.

Lizhen Chen1, Jie Chao2, Xiangmeng Qu1

  • 1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University , 500 Dongchuan Road, Shanghai 200241, PR China.

ACS Applied Materials & Interfaces
|February 22, 2017
PubMed
Summary
This summary is machine-generated.

We developed a novel adenosine triphosphate (ATP) biosensor using a polyA-based aptamer nanobeacon (PAaptNB). This new method offers faster and more sensitive detection of ATP levels in living cells for diagnostic applications.

Keywords:
aptamercellular analysisnanobeaconself-assemblyspherical nucleic acidsurface engineering

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Adenosine triphosphate (ATP) is a vital metabolite essential for numerous cellular functions.
  • Accurate in vivo detection of ATP is critical for advancing diagnostic and theranostic applications.
  • Existing biosensing methods for ATP often lack the required sensitivity and speed.

Purpose of the Study:

  • To develop a highly efficient and rapid biosensor for adenosine triphosphate (ATP) detection.
  • To engineer a polyA-based aptamer nanobeacon (PAaptNB) with tunable properties for ATP analysis.
  • To demonstrate the in vivo applicability of the PAaptNB for cellular ATP imaging.

Main Methods:

  • Construction of a polyA-based aptamer nanobeacon (PAaptNB) with varying polyA lengths.
  • Characterization of dissociation constants and competitive binding kinetics.
  • Evaluation of ATP detection limits and in vivo imaging of intracellular ATP molecules.

Main Results:

  • The polyA length programmably regulated the PAaptNB's binding kinetics and dissociation constants.
  • A detection limit of 10 μM for ATP was achieved with a 30-base polyA length, a ~10-fold improvement over conventional methods.
  • Successful in vivo imaging of intracellular ATP demonstrated the PAaptNB's feasibility for cellular analysis.

Conclusions:

  • The developed PAaptNB offers enhanced efficiency and speed for ATP analysis.
  • Tunable polyA length provides a strategy for optimizing biosensor performance.
  • This novel biosensor holds significant potential for bioanalysis and theranostic applications.