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Optic nerve alterations in PTEN +/- mice.

E Lopez-Sanchez1, E Frances-Muoz, J L Menezo

  • 1Hospital Arnau de Vilanova, Valencia - Spain.

European Journal of Ophthalmology
|February 22, 2017
PubMed
Summary
This summary is machine-generated.

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PTEN gene knockout in mice resulted in larger optic nerves (ON) with hypertrophic axons but no changes in axon number or myelin. This study explored PTEN's role in ON morphology.

Area of Science:

  • Neuroscience
  • Genetics
  • Ophthalmology

Background:

  • The PTEN gene is crucial for regulating cell size, cell cycle arrest, and apoptosis.
  • Optic nerve (ON) morphology is vital for visual function.
  • Understanding PTEN's role can offer insights into optic nerve development and disease.

Purpose of the Study:

  • To investigate the impact of PTEN gene knockout on the morphologic characteristics of the optic nerve.
  • To utilize a mouse model with altered PTEN expression to study optic nerve development and structure.

Main Methods:

  • Morphologic and morphometric analyses of optic nerves from wild-type and heterozygous PTEN knockout mice.
  • Light and electron transmission microscopy were employed for detailed structural examination.

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  • Western blot and immunoblotting were used to assess glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) expression.

    • Main Results:

    • Heterozygous PTEN knockout mice exhibited significantly larger optic nerve cross-sectional areas compared to wild-type controls.
    • Axon diameter was significantly increased in PTEN knockout mice.
    • No significant differences were observed in axon count, intra-axonal degeneration, myelin sheath integrity, or axoplasm density.

    Conclusions:

    • Heterozygous PTEN knockout leads to hypertrophic optic nerves characterized by enlarged axons.
    • Ultrastructural integrity of the optic nerve remains largely unaffected despite PTEN gene alteration.
    • These findings highlight PTEN's role in regulating optic nerve axon and overall nerve size.