Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.7K
Integrins01:10

Integrins

5.9K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
5.9K
Activation of Integrins01:15

Activation of Integrins

5.3K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
5.3K
Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

3.6K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
3.6K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

9.5K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue mechanopathology of cancer.

Developmental cell·2026
Same author

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same author

Tumor Invasive Border Index (TIBI) in colorectal cancer: linking infiltrative morphology to molecular insights.

The Journal of pathology·2026
Same author

Inactive β1-integrin acts as a junctional scaffold for angiopoietin/TIE2/FOXO1 signaling.

The Journal of clinical investigation·2026
Same author

Adhesion-derived condensates control component availability to regulate adhesion dynamics.

Nature communications·2026
Same author

Fibrillar adhesion dynamics govern the timescales of nuclear mechano-response via the vimentin cytoskeleton.

Nature materials·2026

Related Experiment Video

Updated: Mar 7, 2026

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
06:27

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Published on: August 8, 2019

9.7K

Vascular Morphogenesis: An Integrin and Fibronectin Highway.

Hellyeh Hamidi1, Johanna Ivaska2

  • 1Turku Centre for Biotechnology, University of Turku, FIN-20520 Turku, Finland.

Current Biology : CB
|February 22, 2017
PubMed
Summary

Endothelial cells utilize synaptic-like mechanisms for fibronectin secretion and integrin recycling, crucial for vascular development. This discovery sheds light on cellular processes fundamental to blood vessel formation in zebrafish.

More Related Videos

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
07:19

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Published on: December 21, 2016

7.4K
Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
07:52

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

10.6K

Related Experiment Videos

Last Updated: Mar 7, 2026

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
06:27

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Published on: August 8, 2019

9.7K
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
07:19

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

Published on: December 21, 2016

7.4K
Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
07:52

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

10.6K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Vascular Biology

Background:

  • Endothelial cells form blood vessels, a process requiring precise control of extracellular matrix deposition.
  • The mechanisms governing polarized secretion and component recycling in endothelial cells remain incompletely understood.

Purpose of the Study:

  • To investigate the role of synaptic-like machinery in endothelial cell function.
  • To elucidate the process of fibronectin secretion and integrin recycling in vascular development.

Main Methods:

  • Utilized live imaging and genetic manipulation in zebrafish embryos.
  • Investigated the localization and dynamics of fibronectin and integrins using advanced microscopy techniques.

Main Results:

  • Demonstrated that endothelial cells employ synaptic-like machinery for polarized fibronectin secretion.
  • Showed active recycling of integrins to secretion sites, coupled with fibronectin deposition.
  • Confirmed the essential role of this process in zebrafish vascular development.

Conclusions:

  • Endothelial cells possess specialized machinery for directed secretion and matrix assembly.
  • Integrin recycling is dynamically linked to fibronectin deposition, essential for vascular morphogenesis.
  • This study reveals a novel mechanism fundamental to vascular development.