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Updated: Mar 7, 2026
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples
Vinicius Ricardo Acquaro1, Diego Soares Domingues1, Maria Eugênia Costa Queiroz1
1Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
Background:
Polypharmacy is a common practice in schizophrenia. Consequently, therapeutic drug monitoring is usually adopted to maintain the concentrations of the drugs in the plasma within a targeted therapeutic range, to maximize therapeutic efficiency and to diminish adverse side effects.
Methodology:
This study reports on a column switching UHPLC-MS/MS method to determine psychotropic drugs in plasma samples simultaneously.
Results:
The method was linear from 0.025 to 1.25 ng ml-1 with R2 above 0.9950 and the lack of fit test (p > 0.05). The precision values presented coefficients of variation lower than 12%, and the relative standard error of the accuracy were lower than 14%.
Conclusion:
The column switching UHPLC-MS/MS method developed herein successfully determined drugs in schizophrenic patients' plasma samples for therapeutic drug monitoring.
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