Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.5K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.5K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.1K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural color-based microneedle patch with NIR-triggered on-demand drug release for intelligent treatment of infected wounds.

Journal of nanobiotechnology·2026
Same author

Laser Light Scattering-Enhanced Deep Computer Vision Method for the Detection of Trace Mineral Oil in Vegetable Oils.

Analytical chemistry·2026
Same author

A Cd(II) post-modified hydrogen-bonded organic framework toward sensitive uranyl ion detection via turn-off fluorescence.

Talanta·2026
Same author

A Practical DNA Molecular Computing Platform for Identifying Expression Patterns of Multiple miRNAs Related to Diseases.

Analytical chemistry·2026
Same author

Universal Integration of Liquid Crystals with Inorganic Inverse Opals: Robust and Customizable Regulation for Optical Sensing.

Analytical chemistry·2026
Same author

An Integrated Centrifugal Microfluidic Point-of-Care Low-Cost Chip for Rapid Pathogen Nucleic Acid Detection and Diagnosis of Bloodstream Infections.

Analytical chemistry·2026

Related Experiment Video

Updated: Mar 7, 2026

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
08:13

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Published on: February 19, 2016

9.9K

Novel Aptasensor Platform Based on Ratiometric Surface-Enhanced Raman Spectroscopy.

Yan Wu1, Fubing Xiao1, Zhaoyang Wu1

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha, Hunan, China.

Analytical Chemistry
|February 23, 2017
PubMed
Summary

This study introduces a new aptasensor for detecting adenosine triphosphate (ATP) using surface-enhanced Raman scattering (SERS). The novel ratiometric SERS strategy achieves highly sensitive ATP detection with a low limit of detection (20 pM).

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.4K
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
03:33

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

3.4K

Related Experiment Videos

Last Updated: Mar 7, 2026

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
08:13

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Published on: February 19, 2016

9.9K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.4K
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
03:33

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

3.4K

Area of Science:

  • Biosensing
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Adenosine triphosphate (ATP) is a crucial biomarker for various biological processes.
  • Accurate and sensitive detection of ATP is essential for diagnostics and research.
  • Existing ATP detection methods often lack sensitivity or require complex procedures.

Purpose of the Study:

  • To develop a novel aptasensor for quantitative ATP detection.
  • To utilize a ratiometric surface-enhanced Raman scattering (SERS) strategy for enhanced sensitivity.
  • To establish a reliable and generalizable platform for aptasensor development.

Main Methods:

  • Immobilization of thiolated, Rox-labeled complementary DNA (cDNA) onto gold nanoparticles (AuNPs).
  • Hybridization of cDNA with Cy5-labeled ATP-binding aptamer probe to form dsDNA.
  • Ratiometric SERS signal generation based on ATP-induced conformational changes of the aptamer probe.

Main Results:

  • A linear relationship between the ratio of SERS signals (Rox/Cy5) and ATP concentrations (0.1–100 nM).
  • Achieved a low limit of detection (LOD) of 20 pM for ATP.
  • Demonstrated significantly higher sensitivity compared to existing ATP detection methods.

Conclusions:

  • The developed aptasensor platform offers a sensitive and reliable method for quantitative ATP detection.
  • The ratiometric SERS strategy provides a robust approach for biosensing applications.
  • The platform shows potential for adaptation to detect other analytes using aptamer systems.