Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

1.7K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
1.7K
Antigen Processing Pathways01:31

Antigen Processing Pathways

2.7K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
2.7K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phospholipid-driven conformational switching of HCV NS5A links protein folding to replication membrane remodeling.

Science advances·2026
Same author

Single-molecule dynamics reveal ATP binding alone powers substrate translocation by an ABC transporter.

Nature communications·2026
Same author

Braess' Paradox in Enzyme Kinetics: Asymmetry from Population Balance without Direct Cooperativity.

Journal of chemical theory and computation·2026
Same author

Architectural principles of transporter-chaperone coupling within the native MHC I peptide-loading complex.

Science advances·2026
Same author

Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules.

Cell reports·2025
Same author

Engineering Mesoscale T Cell Receptor Clustering by Plug-and-Play Nanotools.

Advanced materials (Deerfield Beach, Fla.)·2024

Related Experiment Video

Updated: Mar 7, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.7K

Proofreading of Peptide-MHC Complexes through Dynamic Multivalent Interactions.

Christoph Thomas1, Robert Tampé1

  • 1Institute of Biochemistry, Biocenter, Goethe University Frankfurt , Frankfurt am Main , Germany.

Frontiers in Immunology
|February 24, 2017
PubMed
Summary
This summary is machine-generated.

The adaptive immune system uses peptide proofreading to select optimal antigens for cell surface presentation. This review details the mechanisms of MHC I chaperones tapasin (Tsn) and TAPBPR in immune recognition.

Keywords:
MHCadaptive immunityantigen presentationmolecular tug-of-warpeptide editingpeptide-loading complexquality controltapasin

More Related Videos

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

15.6K
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.4K

Related Experiment Videos

Last Updated: Mar 7, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.7K
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

15.6K
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.4K

Area of Science:

  • Immunology
  • Molecular Biology
  • Structural Biology

Background:

  • The adaptive immune system identifies and eliminates compromised cells via cytotoxic T lymphocytes recognizing peptides on MHC I molecules.
  • Peptide generation for MHC I presentation occurs in the cytosol, with the peptide-loading complex (PLC) crucial for antigen processing.
  • Key PLC components include the transporter associated with antigen processing (TAP) and tapasin (Tsn), an MHC I-specific chaperone.

Purpose of the Study:

  • To review current knowledge on the molecular mechanisms of peptide proofreading by MHC I chaperones.
  • To present a model for the common catalytic principles of MHC I editors Tsn and TAPBPR, and the MHC II editor HLA-DM.
  • To illustrate distinct quality control strategies employed by these chaperones in epitope sampling.

Main Methods:

  • Biochemical studies
  • Biophysical studies
  • Structural studies
  • Review of existing literature

Main Results:

  • Recent studies have elucidated the catalytic mechanism of Tsn-mediated peptide proofreading.
  • TAP-binding protein-related (TAPBPR) functions as a second MHC I chaperone and peptide editor, independent of the PLC.
  • Both Tsn and TAPBPR likely share common catalytic mechanisms despite differences in allomorph specificity and PLC dependence.

Conclusions:

  • Understanding peptide proofreading catalysis is vital for comprehending immune recognition in infection, cancer, autoimmunity, and transplantation.
  • A unified model highlights shared mechanistic principles among MHC I and MHC II peptide editors.
  • Distinct chaperone strategies ensure robust quality control for immune epitope selection.