Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Surgical Innovation

Background:

• Current cell therapy and tissue engineering often rely on non-autologous materials, posing risks of disease transmission and regulatory challenges.
• Developing patient-derived biomaterials is crucial for enhancing safety and efficacy in regenerative medicine.

Purpose of the Study:

• To introduce a novel, completely intraoperative procedure for generating patient-derived hydrogels.
• To establish a reproducible method for processing peripheral blood into essential biomaterials.
• To present a bedside protocol for preparing autologous hydrogels for clinical applications.

Main Methods:

• Utilized an automated device for processing human peripheral blood to isolate cellular-rich and cellular-poor plasma.
• Engineered a completely autologous hydrogel by substituting calcium for animal-derived thrombin.
• Developed a bedside protocol for hydrogel preparation.

Main Results:

• Successfully created a patient-derived hydrogel using an intraoperative, automated blood processing technique.
• The developed hydrogel is completely autologous, mitigating disease transmission risks.
• The protocol facilitates a reproducible method for biomaterial generation.

Conclusions:

• The described intraoperative procedure offers a safe and efficient method for producing patient-derived hydrogels.
• This approach, termed completely intraoperative tissue engineering strategy (CITES), streamlines the translation of tissue engineering strategies into clinical practice.
• The developed bedside protocol supports the clinical application of autologous hydrogels in cell therapy and regenerative medicine.