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Updated: Mar 7, 2026
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Measurement of hepcidin isoforms in human serum by liquid chromatography with high resolution mass spectrometry
Simon Handley1,2, Lewis Couchman1, Paul Sharp2
1Viapath Analytics, Toxicology Unit, Department of Clinical Biochemistry, King's College Hospital, London SE5 9RS, UK.
Aim:
Hepcidin-25 is the master regulator of iron homeostasis. N-truncated isoforms of hepcidin-25 have been identified (hepcidin-20, -22, -24), although data on the concentrations of these isoforms are sparse.
Materials & Methods:
Serum was mixed with aqueous formic acid, and the supernatant loaded onto a 96-well-SPE-plate. Eluted analytes were analyzed using LC-HR-MS. Forty-seven paired dipotassium-EDTA human plasma and serum samples were analyzed.
Results:
The LLOQ was 1 μg/l (all analytes). Accuracy and precision were acceptable. There was a good correlation (R2 >0.90, all analytes) between matrices. The median (range) serum hepcidin-20, -22, -24 and -25 concentrations measured were 4 (1-40), 8 (2-20), 8 (1-50) and 39 (1-334) μg/l, respectively.
Conclusion:
LC-HR-MS is widely applicable to the measurement of hepcidin-25, and truncated isoforms.
