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Ultraviolet Irradiation Effect on Apple Juice Bioactive Compounds during Shelf Storage
Edmundo Juarez-Enriquez1, Ivan Salmerón2, Nestor Gutierrez-Mendez3
1Centro de Investigación en Alimentación y Desarrollo, Av. Rio Conchos S/N, Parque Industrial, Cd. Cuauhtémoc, Chihuahua 31570, Mexico. edjuen@gmail.com.
Ultraviolet irradiation effectively inactivates enzymes and microbes in apple juice, preserving beneficial antioxidants during storage. This ensures UV-treated apple juice remains a healthy beverage option for consumers.
Area of Science:
- Food Science
- Biotechnology
- Food Microbiology
Background:
- Polyphenol oxidase enzyme and microbial contamination affect apple juice quality and shelf-life.
- Pulsed ultraviolet (UV) light is a non-thermal processing technology with potential for microbial inactivation and enzyme deactivation.
Purpose of the Study:
- To evaluate the efficacy of UV irradiation in inactivating polyphenol oxidase and microbiota in apple juice.
- To assess the impact of UV treatment on bioactive compounds and the stability of apple juice during refrigerated and room temperature storage.
Main Methods:
- Apple juice was treated with UV irradiation at a dose of 345.6 J/cm².
- Evaluated changes in color, antioxidant capacity, polyphenol content, pH, titratable acidity, and total soluble solids.
- Assessed inactivation kinetics of polyphenol oxidase and microbial load during 24 days of storage at 4 °C and 20 °C.
Main Results:
- UV irradiation completely inactivated polyphenol oxidase and microbiota.
- A reduction in antioxidant capacity was observed immediately post-treatment.
- Ascorbic acid content decreased by up to 40% during storage, while total polyphenol content remained stable.
Conclusions:
- UV irradiation is effective for microbial and enzyme inactivation in apple juice.
- UV-treated apple juice maintains its polyphenol content during storage, offering a stable source of antioxidants.
- UV-treated apple juice can be considered a safe and nutritious beverage option.
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