Synergistic effects between a copper-based metal Casiopeína III-ia and cisplatin

Additive, subadditive or superadditive interactions observed in combination therapy play an important role in several treatments, particularly for cancer. The isobolographic analysis allows to establish the pharmacological interactions that exist between two drugs that are administered together in equieffective doses. In order to identify if the combination of two compounds presents synergistic interaction, the antiproliferative activity of CasIII-ia with analogue compounds or cisplatin in different molar ratios was evaluated. Results showed that this compound exhibited additive, subadditive or antagonic and superadditive or synergistic interactions, depending on the compound that accompanies it and the proportion of the compounds in the combination. One of the combinations increased the antiproliferative activity from 50 to 77% when the cells were exposed to 4.59 and 9.70 μM to CasIII-ia and cisplatin, respectively. Further studies of the toxicity and biochemical level of the interactions still remain to be studied on a in-vivo xenographed model.