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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Progress and challenges in electrochemiluminescent aptasensors.

Kateryna Muzyka1, Muhammad Saqib2, Zhongyuan Liu3

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China; Laboratory of Analytical Optochemotronics, Department of Biomedical Engineering, Kharkiv National University of Radio Electronics, Kharkiv 61166, Ukraine.

Biosensors & Bioelectronics
|February 24, 2017
PubMed
Summary

This review explores electrochemiluminescent (ECL) aptasensors, highlighting their features, advances, and applications. It details synergistic benefits, assay designs, and signal amplification for enhanced diagnostic technologies.

Keywords:
AdsorptionAmplificationAptasensorComplementary DNAElectrochemiluminesenceEnergy transferHybridizationIntercalationQuenchingStructure-switching

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • The increasing demand for advanced diagnostic and detection technologies necessitates innovative sensing solutions.
  • Electrochemical luminescence (ECL) offers unique advantages for biosensing due to its inherent characteristics and compatibility with aptamer-based platforms.

Purpose of the Study:

  • To provide a comprehensive review of electrochemiluminescent (ECL) aptasensors.
  • To emphasize the features, advances, challenges, and applications of ECL-based aptasensors.
  • To guide the design and stimulate further development of ECL aptasensors.

Main Methods:

  • Summarizing synergistic benefits of ECL aptamer-based sensors.
  • Classifying ECL aptamer-based assay designs and signal amplification strategies.
  • Highlighting the impact of nanomaterials, immobilization, and detection strategies on analytical performance.

Main Results:

  • Demonstrated the synergistic benefits of integrating ECL with aptasensors.
  • Classified various assay designs and signal amplification techniques for ECL aptasensors.
  • Showcased proof-of-concept studies illustrating the design and application of ECL aptasensors.

Conclusions:

  • ECL aptasensors offer significant potential for sensitive and specific detection.
  • Further research is needed to address remaining challenges and unlock new opportunities.
  • Continued development in nanomaterials and detection strategies will enhance ECL aptasensor performance.