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Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
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Soft tissue engineering with micronized-gingival connective tissues.

Sawako Noda1, Yoshinori Sumita1,2, Seigo Ohba1

  • 1Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Journal of Cellular Physiology
|February 25, 2017
PubMed
Summary

Micronized gingival connective tissue (MGCT) grafts show promise for skin wound healing, promoting vascularization and epithelialization. This micro-grafting technique offers a low-morbidity alternative for keratinized tissue reconstruction.

Keywords:
cell-based therapygingivamicronized-tissueswound healing

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Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Oral Surgery

Background:

  • Free gingival graft (FGG) and connective tissue graft (CTG) are standard for keratinized tissue reconstruction but have donor-site morbidity and limited graft size.
  • Existing methods face challenges with large graft harvesting and achieving adequate recipient site width and thickness.

Purpose of the Study:

  • To investigate the potential of micronized gingival connective tissues (MGCTs) as an alternative for tissue reconstruction.
  • To evaluate the efficacy of MGCT transplantation in promoting skin wound healing.

Main Methods:

  • Micronized gingival connective tissues (MGCTs, ≤100 µm) were prepared from porcine keratinized gingiva using the RIGENERA system.
  • MGCTs were seeded onto an atelocollagen matrix and transplanted into 5 mm full skin defects in immunodeficient mice.
  • Control groups included atelocollagen matrixes with and without micronized dermis.

Main Results:

  • MGCT transplantation significantly enhanced vascularization and epithelialization of the defect area by day 14 compared to controls.
  • Complete wound closure with minimal contraction was observed in the MGCT group after 21 days.
  • Mesenchymal cells from MGCTs survived and potentially contributed to wound healing.

Conclusions:

  • Micronized gingival connective tissue (MGCT) micro-grafting is a viable alternative strategy for keratinized tissue reconstruction.
  • This approach offers reduced donor-site morbidity and readily available graft material.
  • MGCTs promote wound healing through enhanced vascularization, epithelialization, and potential cell survival.