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Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

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Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
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Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

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Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
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Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

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After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
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Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

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Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
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Modified-Release Drug Delivery Systems: Classification01:23

Modified-Release Drug Delivery Systems: Classification

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Modified-release drug delivery systems improve drug efficacy and minimize side effects by controlling the rate and location of drug release. These systems fall into three categories: rate-programmed, stimuli-activated, and site-targeted.Rate-programmed systems release drugs at a predetermined rate, maintaining consistent therapeutic levels and reducing fluctuations that could lead to toxicity or subtherapeutic effects. These systems use polymeric matrices, reservoir-based designs, or osmotic...
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Modified-Release Drug Delivery Systems: Influencing Factors01:20

Modified-Release Drug Delivery Systems: Influencing Factors

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Modified-release drug delivery systems are designed to optimize the therapeutic effect of drugs by minimizing side effects, reducing the dosage required, and controlling drug release to align with pharmacokinetic and pharmacodynamic needs. The system depends on two key factors: the drug's release from the formulation and its movement through the body to the target site. Unlike conventional dosage forms, where absorption is the limiting step, the rate of drug release is the key determinant in...
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Related Experiment Video

Updated: Mar 7, 2026

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Aptamer-based liposomes improve specific drug loading and release.

Kevin Plourde1, Rabeb Mouna Derbali1, Arnaud Desrosiers2

  • 1Faculty of Pharmacy, University of Montreal, QC H3T 1J4, Canada.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|February 28, 2017
PubMed
Summary
This summary is machine-generated.

Aptamers can enhance drug loading into liposomes, significantly improving cancer therapeutics. This study shows aptamers act as multifunctional excipients for better drug delivery and efficacy.

Keywords:
Active loadingAptamerControlled releaseDoxorubicinEncapsulation efficiencyLiposome

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Drug Delivery

Background:

  • Aptamer technology shows promise in cancer therapeutics due to its targeting capabilities.
  • The potential of aptamers to improve drug loading and release from nanocarriers remains underexplored.

Purpose of the Study:

  • To investigate the use of drug-binding aptamers for active drug loading into liposomes.
  • To explore aptamer-based strategies for enhancing drug encapsulation efficiency and controlling drug release.

Main Methods:

  • Designed DNA aptamer sequences specific to doxorubicin with varying binding affinities.
  • Incorporated aptamers into cationic liposomes to facilitate active drug loading.
  • Optimized liposome formulations for drug/aptamer ratios and charge to maximize encapsulation efficiency.
  • Evaluated drug release kinetics and cytotoxicity on HeLa cells.

Main Results:

  • Aptamer incorporation into liposomes preserved binding ability, enabling active doxorubicin loading.
  • Achieved ≥80% doxorubicin encapsulation efficiency, significantly higher than passive methods.
  • Demonstrated that aptamer structure influences drug release and therapeutic efficacy.
  • Successfully improved the loading of tobramycin, a hydrophilic drug, six-fold using aptamers.

Conclusions:

  • Aptamers can function as multifunctional excipients in liposomal formulations, enhancing drug loading and release.
  • Aptamers with specific intermediate binding affinity are optimal for high drug loading and controlled release.
  • This aptamer-based strategy offers a promising approach for improving nanocarrier-based drug delivery systems.