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Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells
Umesh D Wankhade1, Sushil G Rane2
1Cell Growth and Metabolism Section, Diabetes, Endocrinology, and Obesity Branch, NIDDK, National Institutes of Health, Clinical Research Center, West Laboratories 5-5940, 10 Center Dr., Bethesda, MD, 20892, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 1, 2017
Summary
Understanding adipose tissue progenitors is key to metabolic health. Identifying these cells will help unravel the roles of white, brown, and beige adipocytes in obesity and diabetes.
Area of Science:
- Metabolic disease research
- Cell biology
- Adipogenesis
Background:
- Adipose tissue dysfunction is central to metabolic diseases like obesity and diabetes.
- White adipocytes store fat, while brown adipocytes dissipate energy through thermogenesis.
- Beige adipocytes, inducible within white fat, also contribute to thermogenesis.
Purpose of the Study:
- To identify progenitor cells that can differentiate into white, brown, and beige adipocytes.
- To enable functional characterization of these distinct adipocyte subtypes.
- To advance understanding of adipogenesis in metabolic health and disease.
Main Methods:
- The study focuses on identifying and isolating adipocyte progenitors.
- Methods aim for robustness, reliability, and reproducibility.
- Investigates the origins of white, brown, and beige adipocytes from stem/precursor cells or via transdifferentiation.
Main Results:
- The abstract suggests that white, brown, and beige adipocytes can arise from common progenitors.
- The presence and type of adipocytes correlate with metabolic dysfunction.
- Identification of progenitors is crucial for understanding adipocyte formation.
Conclusions:
- Identifying adipocyte progenitors is critical for understanding metabolic diseases.
- Robust methods for progenitor isolation are needed to study white, brown, and beige adipogenesis.
- This research has significant medical relevance for metabolic health.
