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Updated: Mar 7, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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A fully automated flow-based approach for accelerated peptide synthesis.

Alexander J Mijalis1, Dale A Thomas2, Mark D Simon1

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Nature Chemical Biology
|March 1, 2017
PubMed
Summary

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A new automated flow synthesis method drastically speeds up solid-phase polypeptide synthesis. This rapid peptide synthesis achieves high purity and yield, enabling mass production of peptides.

Area of Science:

  • Biochemistry and Organic Chemistry
  • Chemical Engineering
  • Synthetic Biology

Background:

  • Solid-phase peptide synthesis (SPPS) is a cornerstone of peptide production.
  • Traditional SPPS methods can be time-consuming and labor-intensive.
  • There is a need for faster, more efficient peptide synthesis techniques.

Purpose of the Study:

  • To develop a fully automated, flow-based approach for solid-phase polypeptide synthesis.
  • To significantly reduce synthesis time per amino acid residue.
  • To achieve high purity and yield comparable to conventional methods.

Main Methods:

  • Implementation of a flow-based system for automated solid-phase polypeptide synthesis.
  • Optimization of amide bond formation within the flow system.

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Last Updated: Mar 7, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

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  • Characterization of crude peptide purity and isolated yields.

    • Main Results:

    • Achieved amide bond formation in as little as 7 seconds.
    • Total synthesis time reduced to 40 seconds per amino acid residue.
    • Crude peptide purities and isolated yields were comparable to standard batch SPPS.

    Conclusions:

    • The developed automated flow-based synthesis is a highly efficient method for polypeptide production.
    • This approach enables rapid synthesis of peptides with high purity and yield.
    • The system has the potential for large-scale production, yielding tens of thousands of peptides annually.