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Updated: Mar 7, 2026
Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
[Establishment and Application of a Method for Determination of Glycosyltransferase Activity]
Yan-Lian Liang1, Yu-Qing Su1, Yin-Ze Zhang1
1Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
Objective:
To establish a method for determination of glycosyltransferase and to explore the enzyme A, B glycosyltransferase activity in human serum so as to lay the foundation for the determination of enzyme level and enzyme activity.
Methods:
The glycosyltransferase activity kit was used to draw phosphate standard curves in our laboratory. The A and B glycosyltransferase activity were determined by the standard curves.
Results:
The standard curves (y=2671.3x-0.596 R2=0.9998) for determing glycosyltransferase activity suitable for use in our laboratory were drawn. At the same time the method was set up for determination of A, B glycosyltransferase in human serum.
Conclusion:
The establised method of the determination of glycosyltransferase is suitable for common type of enzyme activity and suitable for the A, B glycosyltransferase in human serum.
