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Flow effects on the viability and lysis of suspended mammalian cells
Anne McQueen1, Eliane Meilhoc1, James E Bailey1
1Department of Chemical Engineering, California Institute of Technology, 91125, Pasadena, California, USA.
Abstract:
A mouse myeloma cell line growing in suspension was subjected intermittently to flow through a sudden contraction and turbulent flow in a capillary tube. The probability of lysis per pass through the capillary tube increased with average wall shear stress level and with residence time per pass in the tube. Lysis was first observed at a threshold average wall shear stress level of 1800 dyn/cm2. Although the flow caused lysis, it had no effect on cell viability.

