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Characterization of the purified multifunctional cellulase component of Penicillium funiculosum
N A Sahasrabudhe1, A H Lachke1, P K Ranjekar1
1Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India.
Abstract:
The endoglucanase component (CMCase I) ofPenicillium funiculosum cellulase was purified to apparent homogeneity by ultrafiltration and gel chromatography. It consists of a single polypeptide chain with a molecular weight of 56000 and is a glycoprotein. Viscometric and end-product analysis revealed the randomness of enzyme action. Multifunctional characteristic of CMCase I was studied with various carbohydrate substrates.
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