Related Experiment Video
Updated: Mar 7, 2026

Methodology for Sputum Induction and Laboratory Processing
Published on: December 17, 2017
Sputum collection and analysis: what the nurse needs to know
1Guy's Hospital, Guy's and St Thomas' NHS Foundation Trust, London, England.
Abstract:
Rationale and key points Sputum is the term used to describe mucus that has been expectorated. It consists of secretions and other matter that has been coughed up from the lungs and large airways. This article informs nurses about how and why sputum collection and analysis are undertaken. » Sputum is always considered abnormal, because healthy people swallow bronchial secretions. » Sputum analysis is important, because it enables diagnosis of conditions such as pneumonia, tuberculosis, lung abscess and lung cancer. » The nurse should record the colour, amount and consistency of the sputum collected. They should send the specimen to the laboratory for analysis without delay to ensure accurate results.
Related Concept Videos
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies
Medical History
Pulmonary Function Tests
Pulmonary Function Tests are crucial diagnostic tools for assessing respiratory function, particularly in patients with chronic respiratory disorders. They comprehensively evaluate lung volumes, ventilatory function, breathing mechanics, diffusion, and gas exchange. These tests help diagnose pulmonary diseases and play a significant role in monitoring disease progression, evaluating disability, and assessing response to therapy.
PFTs involve using a spirometer, a...
Blood Studies I: ABG and VBG
Arterial Blood Gas (ABG)
Arterial Blood Gas (ABG) studies are crucial for assessing the lungs' ability to supply oxygen and remove carbon dioxide, reflecting the patient's ventilation status. They also help understand the kidneys' capacity to...
Sample Preparation for Analysis: Overview
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

