Elementary Protein Analysis in Schizosaccharomyces pombe
Agnes Grallert1, Iain M Hagan1
1CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.uk.
Cold Spring Harbor Protocols
|March 3, 2017
Abstract:
Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability make Schizosaccharomyces pombe an excellent model organism in which to use mass spectrometry to conduct proteome-wide approaches. Here we discuss issues encountered during the analysis of fission yeast protein preparations.
