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Osteogenesis evaluation of duck's feet-derived collagen/hydroxyapatite sponges immersed in dexamethasone
Yeon Ji Kook1, Dae Hoon Lee1, Jeong Eun Song1
1Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer Fusion Research Center, Chonbuk National University, 567, Beackje-daero, Deokjin, Jeonju, 561-756 Republic of Korea.
Background:
The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone.
Methods:
The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(-)DC and Dex(-)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(-)DC, Dex(-)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR).
Results:
In this study, we assessed osteogenic differentiation of BMSCs on Duck's feet-derived collagen (DC)/HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days.
Conclusion:
From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.
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