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Updated: Mar 6, 2026
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
A novel microRNA-1207-3p/FNDC1/FN1/AR regulatory pathway in prostate cancer
Dibash K Das1, Olorunseun O Ogunwobi1
1Department of Biological Sciences, Hunter College of The City University of New York, New York, NY, 10065, USA; The Graduate Center Departments of Biology and Biochemistry, The City University of New York, New York, NY, 10016, USA; Department of Medicine, Weill Cornell Medicine, Cornell University, New York, NY, 10065, USA.
Abstract:
Prostate cancer (PCa) is the second most common cause of cancer-specific deaths in the U.S. Unfortunately, the underlying molecular mechanisms for its development and progression remain unclear. Studies have established that microRNAs (miRNAs) are dysregulated in PCa. The intron-derived microRNA-1207-3p (miR-1207-3p) is encoded at the non-protein coding gene locus PVT1 on the 8q24 human chromosomal region, an established PCa susceptibility locus. However, miR-1207-3p in PCa had not previously been investigated. Therefore, we explored if miR-1207-3p plays any regulatory role in PCa. We discovered that miR-1207-3p is significantly underexpressed in PCa cell lines in comparison to normal prostate epithelial cells, and that increased expression of microRNA-1207-3p in PCa cells significantly inhibits proliferation, migration, and induces apoptosis via direct molecular targeting of fibronectin type III domain containing 1 (FNDC1). Our studies also revealed significant overexpression of FNDC1, fibronectin (FN1) and the androgen receptor (AR) in human PCa cell lines as well as tissues, and FNDC1, FN1, and AR positively correlate with aggressive PCa. These findings, recently published in Experimental Cell Research, are the first to describe a novel miR-1207-3p/FNDC1/FN1/AR novel regulatory pathway in PCa.
Insights
MicroRNA-1207-3p is underexpressed in prostate cancer (PCa). Increasing its expression inhibits PCa cell growth and migration by targeting FNDC1, revealing a new regulatory pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Prostate cancer (PCa) is a leading cause of cancer deaths, with unclear molecular mechanisms.
- MicroRNAs (miRNAs) are known to be dysregulated in PCa.
- The 8q24 locus, containing the PVT1 gene, is a known PCa susceptibility region.
Purpose of the Study:
- To investigate the role of microRNA-1207-3p (miR-1207-3p) in PCa.
- To identify potential molecular targets of miR-1207-3p in PCa.
- To explore a novel regulatory pathway involving miR-1207-3p in PCa progression.
Main Methods:
- Comparison of miR-1207-3p expression in PCa cell lines versus normal prostate cells.
- Assessing the effect of miR-1207-3p overexpression on PCa cell proliferation, migration, and apoptosis.
- Identifying direct molecular targets of miR-1207-3p using bioinformatics and experimental validation.
- Analyzing the expression of FNDC1, FN1, and AR in PCa cell lines and tissues.
Main Results:
- miR-1207-3p is significantly underexpressed in PCa cell lines.
- Overexpression of miR-1207-3p inhibits PCa cell proliferation and migration, and induces apoptosis.
- Fibronectin type III domain containing 1 (FNDC1) was identified as a direct molecular target of miR-1207-3p.
- FNDC1, fibronectin (FN1), and androgen receptor (AR) are overexpressed in PCa and correlate with aggressive disease.
Conclusions:
- miR-1207-3p acts as a tumor suppressor in PCa.
- The novel miR-1207-3p/FNDC1/FN1/AR pathway is implicated in PCa development and progression.
- Targeting this pathway may offer new therapeutic strategies for PCa.
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