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Published on: July 21, 2016
Maternal-Child Microbiome: Specimen Collection, Storage, and Implications for Research and Practice
Sheila Jordan1, Brenda Baker, Alexis Dunn
1Sheila Jordan, MPH, RN, is Doctoral Student; Brenda Baker, PhD, RNC, CNS, is Assistant Professor; Alexis Dunn, MSN, CNM, is Doctoral Candidate; Sara Edwards, CNM, MN, MPH, is Instructor and Doctoral Candidate; Erin Ferranti, PhD, MPH, RN, is Assistant Professor; Abby D. Mutic, MSN, CNM, is Doctoral Student; Irene Yang, PhD, RN, is Assistant Professor; and Jeannie Rodriguez, PhD, MSN, RN, C-PNP, is Assistant Clinical Professor, Nell Hodgson Woodruff School of Nursing, Emory University, Atlanta, Georgia.
Proper collection and storage of maternal and child microbiome samples are crucial for accurate research. Adhering to specific protocols ensures reliable data for improving maternal and infant health outcomes.
Area of Science:
- Microbiome research
- Maternal and child health
- Nursing science
Background:
- The maternal microbiome significantly influences pregnancy outcomes and the health of mother and infant.
- Advances in maternal and child microbiome science offer potential solutions for pregnancy complications and chronic conditions.
Purpose of the Study:
- To review site-specific considerations for collecting and storing maternal and child microbiome samples.
- To discuss the implications for nursing research and practice.
Main Methods:
- A review and synthesis of microbiome sampling protocols.
- Identification of precautions across various sampling methods.
Main Results:
- Viable sampling sites include oral, vaginal, gut, placental, and breast milk.
- Pre-sampling considerations include medications, health status, and hygiene.
- Proper storage is essential to prevent sample degradation.
Conclusions:
- Microbiome changes during perinatal, postpartum, and childhood periods significantly impact long-term health.
- Accurate sampling techniques are vital for evidence-based practice.
- Nurses play a key role in microbiome research and clinical application.
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Microbial Classification System
Key Techniques in Microbiology
Sample Handling
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...

