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Updated: Mar 6, 2026

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Imaging Vesicular Traffic at the Immune Synapse.

Jérôme Bouchet^1,2, Iratxe Del Río-Iñiguez^1,2, Andrés Alcover^3,4

¹Department of Immunology, Lymphocyte Cell Biology Unit, Institut Pasteur, 25-28 Rue du Docteur Roux, Paris, 75015, France.

Methods in Molecular Biology (Clifton, N.J.)

|March 4, 2017

Summary

T cell polarization involves cytoskeletal dynamics and vesicle transport. This study details microscopy methods to visualize how T cell receptor (TCR) and signaling molecules traffic via endosomes to the immunological synapse.

Keywords:

Cytoskeleton Endosomes Immunological synapse T cell activation TCR signaling Vesicle traffic

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Last Updated: Mar 6, 2026

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

Published on: December 24, 2015

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

Area of Science:

Immunology
Cell Biology
Microscopy

Background:

Immunological synapse formation requires T cell polarization.
Cytoskeletal dynamics and intracellular vesicle traffic are crucial for this process.
Endosomal vesicle traffic delivers T cell receptor (TCR) and signaling molecules to the synapse.

Purpose of the Study:

To describe microscopy imaging methods for studying T cell polarization.
To investigate the association of TCR and signaling molecules with endosomal compartments.
To understand the delivery of cargo to the immunological synapse.

Main Methods:

Advanced microscopy techniques were employed.
Imaging focused on the T cell polarization process.
Methods were developed to visualize endosomal vesicle traffic.

Main Results:

The study visualizes the association of TCR and signaling molecules with endosomes.
Methods demonstrate the delivery of cargo to the immunological synapse.
Insights into the dynamics of T cell receptor trafficking were gained.

Conclusions:

Microscopy imaging provides critical insights into T cell polarization.
Endosomal vesicle traffic plays a key role in immunological synapse formation.
The described methods enable further investigation of T cell signaling complex assembly.