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Updated: Jun 20, 2026

Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number

Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number

Published on: November 16, 2010

Image processing approaches for microtubule remodeling quantification at the immunological synapse.

Daniel Krentzel¹, Maria Isabella Gariboldi¹, Marie Juzans²

¹Institut Pasteur, Université Paris Cité, CNRS-UMR3691, Unité Imagerie et Modélisation, Paris, France.

Methods in Cell Biology

|February 7, 2025

Summary

Researchers developed a new image analysis method to quantify microtubule organization in T cell immunological synapses. This tool aids in understanding T cell functions and potential roles in anti-tumor immunity.

Keywords:

Adenomatous polyposis coli Image processing Immunological synapse Microtubule remodeling Open-source napari plugin Polarity regulators Quantitative image analysis T cells

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Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

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Last Updated: Jun 20, 2026

Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number

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Published on: November 16, 2010

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

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Published on: April 9, 2018

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Area of Science:

Immunology
Cell Biology
Biophysics

Background:

Immunological synapses form via T cell polarization, requiring extensive cytoskeleton remodeling.
Actin and microtubules are critical for synapse architecture, organelle positioning, and T cell effector functions.
Quantifying complex filamentous networks in synapses is challenging due to their heterogeneous nature.

Purpose of the Study:

To develop an image processing approach for quantifying microtubule organization at the immunological synapse without filament segmentation.
To investigate the role of Adenomatous polyposis coli (Apc) in immunological synapse structure and function.
To explore the implications of Apc in anti-tumor immune responses.

Main Methods:

Developed a novel image processing method analyzing spatial and directional organization of microtubules.
The method quantifies microtubule organization emanating from the centrosome to the synapse periphery.
An open-source napari plugin was created for analyzing filamentous networks.

Main Results:

Successfully quantified microtubule organization at the immunological synapse using the developed image processing approach.
Demonstrated the utility of the method in studying the role of Adenomatous polyposis coli (Apc).
Provided a tool for researchers to analyze cytoskeletal dynamics in T cell synapses.

Conclusions:

The new image processing method enables robust quantification of microtubule organization in immunological synapses.
This approach facilitates the study of polarity regulators like Apc and their impact on T cell function.
Understanding cytoskeletal dynamics in synapses is crucial for deciphering T cell-mediated immunity and potential therapeutic strategies.