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T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Related Experiment Video

Updated: Mar 6, 2026

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Published on: March 25, 2016

Imaging Asymmetric T Cell Division.

Mirren Charnley^1,2,3, Sarah M Russell^4,5,6,7

¹Faculty of Science, Engineering and Technology, Centre for Micro-Photonics, Swinburne University of Technology, Mail No H74, PO Box 218, Hawthorn, VIC, 3122, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|March 4, 2017

Summary

This summary is machine-generated.

Asymmetric cell division (ACD) is crucial for T cell fate and hematopoiesis. This study details methods for monitoring and quantifying ACD in developing and activated T cells, addressing current challenges in T cell research.

Keywords:

Asymmetric cell division Cell fate determinants Cell polarity Fluorescence microscopy Image quantification Lymphocytes T Cells Thymocyte

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Last Updated: Mar 6, 2026

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Published on: March 25, 2016

Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

Imaging the Human Immunological Synapse

Imaging the Human Immunological Synapse

Published on: December 26, 2019

Area of Science:

Immunology
Developmental Biology
Cell Biology

Background:

Asymmetric cell division (ACD) is a fundamental process regulating cell fate in model organisms.
ACD is increasingly recognized for its role in T cell development and hematopoiesis.
Standardized methods for assessing ACD in T cells are still under development.

Purpose of the Study:

To describe current methods for monitoring and measuring ACD in developing and activated T cells.
To provide a comprehensive overview of techniques for capturing T cells undergoing cytokinesis.
To discuss approaches for quantifying ACD in lymphocytes and address interpretation challenges.

Main Methods:

Overview of in vivo, ex vivo, and in vitro methods for capturing cells during cytokinesis.
Detailed protocols for in vitro fixed immunofluorescent staining.
Time-lapse microscopy and analysis for dynamic ACD monitoring.
Approaches for quantifying ACD in lymphocytes, including potential pitfalls.

Main Results:

Established a framework for assessing ACD in T cells.
Highlighted various techniques applicable to different experimental settings (in vivo, ex vivo, in vitro).
Provided detailed guidance on quantification and interpretation of ACD in T cell populations.

Conclusions:

Accurate assessment of ACD is critical for understanding T cell differentiation and function.
The described methods offer a valuable resource for researchers studying T cell biology.
Standardization of ACD measurement will advance the field of T cell hematopoiesis research.