Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules
Neeraj Kohli1, Melissa L Geddie2
1Merrimack Pharmaceuticals, Inc., Suite B7201, 1 Kendall Square, Cambridge, MA, 02139, USA. nkohli@merrimack.com.
Screening for high-affinity antibodies is common, but finding candidates with good biophysical properties remains difficult. This study introduces a simple HPLC method to assess antibody developability early in the discovery pipeline.
Area of Science:
- Biochemistry
- Biotechnology
- Pharmaceutical Sciences
Background:
- High-affinity antibody discovery is a standard procedure in modern biotechnology.
- Assessing biophysical characteristics alongside biological activity is crucial but challenging for antibody candidates.
Purpose of the Study:
- To present a straightforward high-performance liquid chromatography (HPLC)-based method for screening antibody developability.
- To enable earlier assessment of critical developability factors during the antibody discovery process.
Main Methods:
- Development of a simple HPLC-based assay.
- Integration of the assay into the early antibody discovery workflow.
Main Results:
- The HPLC method provides a means to evaluate developability factors.
- Early screening allows for faster identification of suitable antibody candidates.
Conclusions:
- The described HPLC method simplifies the assessment of antibody developability.
- Implementing this screening approach can accelerate the discovery of robust antibody therapeutics.
More Related Videos
09:45Author Spotlight: Detecting Low-Abundant Host Cell Proteins in Drug Products Using Enrichment Beads and Limited Digestion
Published on: January 19, 2024
13:59High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds
Published on: August 18, 2023