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Cell and Tissue Imaging with Molecularly Imprinted Polymers.

Maria Panagiotopoulou1, Stephanie Kunath2, Karsten Haupt2

  • 1CNRS Enzyme and Cell Engineering Laboratory, Sorbonne Universités, Université de Technologie de Compiègne, Rue Roger Couttolenc, CS 60319, Compiègne Cedex, 60203, France. maria.panagiotopoulou@utc.fr.

Methods in Molecular Biology (Clifton, N.J.)
|March 4, 2017
PubMed
Summary

Molecularly imprinted polymers (MIPs) act as artificial antibodies for precise cell imaging. This study successfully used MIP nanoparticles to detect cancer and infection biomarkers like hyaluronan and sialylation on human cells and tissues.

Keywords:
Artificial antibodiesCell imagingGlucuronic acidHyaluronic acidMIPsMolecularly imprinted polymersMultiplexed imagingQuantum dotsSialylationTissue imaging

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cell Biology

Background:

  • Advanced cell imaging tools are crucial for detecting cancer and infection biomarkers.
  • Targeting these biomarkers is difficult due to a lack of specific receptor materials.
  • Molecularly imprinted polymers (MIPs) offer a solution as tailored, stable artificial receptors.

Purpose of the Study:

  • To demonstrate the use of MIPs as artificial antibodies for selective cell labeling and imaging.
  • To target specific biomarkers such as hyaluronan and sialylation moieties.
  • To develop a generalizable approach for molecular imaging in cells and tissues.

Main Methods:

  • Synthesized fluorescently labeled MIP nanoparticles (MIPGlcA and MIPNANA) using glucuronic acid and N-acetylneuraminic acid.
  • Applied rhodamine-labeled MIPGlcA particles (~400 nm) to target extracellular hyaluronan.
  • Utilized MIP-coated InP/ZnS quantum dots (QDs, ~125 nm) for multiplexed imaging of intra- and extracellular hyaluronan and sialylation sites.

Main Results:

  • Successfully achieved selective labeling and imaging of hyaluronan and sialylation on human skin cells and tissues.
  • Demonstrated multiplexed imaging using differently colored QDs functionalized with MIPGlcA and MIPNANA.
  • Showcased the ability of MIPs to act as effective artificial antibodies for specific molecular targets.

Conclusions:

  • MIPs serve as versatile artificial antibodies for specific molecular recognition and imaging in biological samples.
  • This approach enables precise detection and localization of biomarkers associated with cancer and infection.
  • The methodology is adaptable for targeting a broader range of molecules within cells and tissues.