Related Experiment Video
Updated: Mar 6, 2026
09:08
Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies
Published on: June 2, 2021
2.1K
Comprehensive bioinformatics analyses of Crohn's disease
Yi Zhou1, Cheng Zhan2, Yingyu Huang1
1Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.
Molecular Medicine Reports
|March 6, 2017
Summary
This study analyzed gene expression in Crohn's disease (CD) to identify key biological pathways and potential biomarkers. Researchers found 432 differentially expressed genes and specific microRNAs that could lead to new CD diagnostics and treatments.
Area of Science:
- Gastroenterology
- Immunology
- Bioinformatics
Background:
- Crohn's disease (CD) is a chronic inflammatory condition with rising global incidence.
- Microarray data analysis offers insights into the complex pathobiology of CD.
Purpose of the Study:
- To comprehensively analyze gene expression data in CD using bioinformatics.
- To identify differentially expressed genes (DEGs), enriched pathways, and regulatory microRNAs (miRNAs) in CD.
Main Methods:
- Utilized Gene Expression Omnibus database for CD colon tissue gene expression data.
- Performed differential gene expression analysis, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses.
- Employed TargetScan and miRDB for miRNA-gene interaction prediction.
Main Results:
- Identified 432 DEGs (229 upregulated, 203 downregulated) in CD samples, including MMP3 and GSTA1.
- DEGs are significantly involved in inflammatory response, immune response, cell migration, and JAK/STAT signaling.
- miR-149-3p and miR-4447 were predicted as key regulators of identified DEGs.
Conclusions:
- The study elucidates molecular mechanisms underlying CD pathogenesis.
- Identified DEGs and regulatory miRNAs represent potential diagnostic biomarkers and therapeutic targets for Crohn's disease.
Related Concept Videos
01:30Inflammatory Bowel Disease II: Crohn's Disease
1.4K
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
1.4K
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
987
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
987
01:11Genome-wide Association Studies-GWAS
16.2K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
16.2K
02:02Genomics
41.2K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
41.2K
01:24Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
670
Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...
670
01:29Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
618
Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...
618