Related Experiment Video

Updated: Mar 6, 2026

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.9K

Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer

Jingjing Tian1, Xibao Hu2, Wei Gao1

  • 1Department of Gastroenterology, NanKai Hospital, Tianjin 300100, P.R. China.

Molecular Medicine Reports
|March 6, 2017
PubMed

Insights

LET long non-coding RNA (lncRNA) is downregulated in gastric cancer, acting as a tumor suppressor. Restoring LET inhibits cancer cell proliferation and migration, suggesting its potential as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) regulate biological processes.
  • Altered lncRNA expression contributes to cancer development and metastasis.
  • The role of LET lncRNA in gastric cancer is currently unknown.

Purpose of the Study:

  • To investigate the expression levels of LET in gastric cancer.
  • To elucidate the function of LET in gastric cancer progression.
  • To evaluate LET as a potential biomarker or therapeutic target for gastric cancer.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to detect LET expression in tissues and cell lines.
  • Cell counting kit-8, wound scratch, and ELISA assays to assess cell proliferation, migration, and apoptosis.
  • Transfection of gastric cancer cells with a LET recombinant overexpression vector.

Main Results:

  • LET expression was significantly downregulated in gastric cancer tissues and cell lines compared to normal controls.
  • Overexpression of LET in gastric cancer cells inhibited proliferation and migration.
  • Restoration of LET expression promoted apoptosis in gastric cancer cells in vitro.

Conclusions:

  • LET functions as a tumor suppressor in gastric cancer.
  • LET downregulation is associated with gastric cancer development.
  • LET represents a potential diagnostic biomarker and therapeutic target for gastric cancer.

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.2K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.0K