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Reduced expression of MHC class I antigens in chicken fibroblasts transformed by Rous sarcoma virus

J Gogusev1, G Suskind, D Gilmour

  • 1Laboratoire de Médecine expérimentale, Collège de France, Paris.

Folia Biologica
|January 1, 1987
PubMed

Insights

Rous sarcoma virus (RSV) transformation reduces H-B class I antigen expression in chicken fibroblasts. Avian leukosis virus (ALV) infection did not alter these antigen levels, suggesting specific viral effects on cell surface molecules.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Chicken embryo fibroblasts express H-B class I antigens, crucial for immune recognition.
  • Rous sarcoma virus (RSV) is known to transform cells, potentially altering cell surface protein expression.
  • Different chicken inbred lines exhibit varying baseline levels of H-B antigens.

Purpose of the Study:

  • To investigate the effect of Rous sarcoma virus (RSV) transformation on H-B class I antigen expression in chicken fibroblasts.
  • To compare antigen expression in RSV-transformed cells versus uninfected and avian leukosis virus (ALV)-infected cells.
  • To assess the influence of genetic background (inbred lines) on H-B antigen levels.

Main Methods:

  • Utilized monoclonal and polyclonal antibodies against H-B class I (B-F) antigens.
  • Analyzed antigen expression on normal and in vitro transformed chick embryo fibroblasts.
  • Quantified antigen levels at the cell membrane and in whole cell lysates using techniques like flow cytometry or Western blotting (implied).

Main Results:

  • RSV-transformed fibroblasts (SR-RSV-B) showed significantly reduced H-B class I antigen expression compared to uninfected controls.
  • This reduction was observed both at the cell membrane and within whole cell lysates.
  • Chick embryo fibroblasts infected with avian leukosis virus (RAV-1) exhibited H-B class I antigen levels comparable to control cells.
  • Normal fibroblasts from B4/B4 inbred line expressed higher H-B antigen levels than those from the B12/B12 inbred line.

Conclusions:

  • In vitro transformation by Schmidt-Ruppin strain Rous sarcoma virus of subgroup B (SR-RSV-B) leads to a quantitative decrease in H-B class I antigen expression on chicken fibroblasts.
  • Avian leukosis virus (RAV-1) infection does not significantly alter H-B class I antigen levels, indicating specificity in viral-induced changes.
  • Genetic background influences H-B antigen expression, with B4/B4 lines showing higher levels than B12/B12 lines.

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