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Related Experiment Video

Updated: Mar 6, 2026

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
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Quantitative phase imaging for cell culture quality control.

Lena Kastl1, Michael Isbach1, Dieter Dirksen2

  • 1Biomedical Technology Center, University of Muenster, Mendelstr. 17, Muenster, D-48149, Germany.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|March 7, 2017
PubMed
Summary
This summary is machine-generated.

Quantitative phase imaging (QPI) using digital holographic microscopy (DHM) offers label-free cell analysis. This method quantifies cell parameters for improved cell culture quality assessment with minimal sample preparation.

Keywords:
cell culture qualitydigital holographic microscopyimaging cytometrylive cell imagingquantitative phase imaging

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Cell culture quality is crucial for research and biopharmaceutical production.
  • Current methods for cell quality assessment often require labeling or extensive sample preparation.
  • Quantitative Phase Imaging (QPI) offers a label-free approach to cell analysis.

Purpose of the Study:

  • To explore the potential of QPI with digital holographic microscopy (DHM) for quantifying cell culture quality.
  • To analyze cellular parameters like refractive index, volume, and dry mass.
  • To establish QPI as a method for label-free imaging cytometry.

Main Methods:

  • Label-free QPI of detached single cells in suspension using Michelson interferometer-based self-interference DHM.
  • Analysis of two pancreatic tumor cell lines under varying culture conditions.
  • Quantification of cellular response to medium osmolality changes.

Main Results:

  • Identified adequate cell numbers for reliable statistical analysis.
  • Demonstrated reproducibility of QPI measurements.
  • Showed that QPI image evaluation extracts absolute cell parameters related to cell layer confluence.

Conclusions:

  • QPI combined with DHM enables label-free imaging cytometry.
  • Provides novel, complementary biophysical data for sophisticated cell culture quality quantification.
  • Minimizes sample preparation requirements for cell analysis.