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Updated: Mar 6, 2026

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Relationship between Structure and Fluctuations of Lipid Nonlamellar Phases Deposited at the Solid-Liquid Interface
Tommy Nylander1,2, Olaf Soltwedel3,4, Marina Ganeva5
1Physical Chemistry, Department of Chemistry, Lund University , P.O. Box 124, SE-22100 Lund, Sweden.
Abstract:
The structure and dynamics of nanostructure films formed by mixtures of soy phosphatidylcholine and glycerol dioleate at the silicon-aqueous interface were studied by grazing incidence neutron spin echo spectroscopy (GINSES), specular and off-specular neutron reflectometry, and small-angle X-ray diffraction. Reverse hexagonal (HII) and micellar cubic phase (Fd3m) layers at the solid-liquid interface have been identified with neutron reflectometry measurements. A preferred orientation of the liquid crystalline (LC) domains was observed only for the anisotropic HII phase. The size of the LC domains was found to be about 1 micrometer as estimated from the width of the diffraction peaks. GINSES revealed that the cubic phase forms rather rigid films. In comparison, the HII film was more flexible, appearing as a modified undulation spectrum of the cylinders due to the interaction with the substrate.
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