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A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets
Alais Maria Dall Agnol1, Rodrigo Alejandro Arellano Otonel2, Raquel Arruda Leme1
1Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil; Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, PR CEP 86057-970, Brazil.
Abstract:
This study describes a sensitive (1.3 × 101 genomic copies/μL) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.
