Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.8K
Computed Tomography01:10

Computed Tomography

9.2K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
9.2K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.6K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

514
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
514
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using Principal Components Analysis to Visualize Motion and Mitigate Artifacts in Dynamic Optical Coherence Tomography.

Journal of biophotonics·2026
Same author

CeraMIRScan: Mid-infrared OCT Scan Dataset for Ceramic Quality Assessment.

Scientific data·2026
Same author

Time-domain optical coherence tomography at 2 µm using GaSb-based broadband superluminescent diode.

Optics express·2026
Same author

Ultra-low-noise supercontinuum in normal-dispersion ZBLAN fibers pumped at 1.85 µm.

Optics letters·2026
Same author

Exploiting wave breaking asymmetry for broadband cascaded ANDi-supercontinuum generation.

Optics letters·2025
Same author

Investigating phototoxicity of optical coherence tomography imaging in porcine and human spermatozoa.

Reproductive biomedicine online·2025

Related Experiment Video

Updated: Mar 6, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

9.0K

Gabor fusion master slave optical coherence tomography.

Ramona Cernat1, Adrian Bradu1, Niels Møller Israelsen2

  • 1Applied Optics Group, School of Physical Sciences, University of Kent, Canterbury CT2 7NH, Kent, UK.

Biomedical Optics Express
|March 9, 2017
PubMed
Summary

This study applies Gabor filtering to a Master/Slave (MS) swept source optical coherence tomography (SS-OCT) system, enabling dynamic focusing and depth-specific signal retrieval for enhanced imaging. The combined MS-OCT and Gabor filtering approach offers faster volume assembly compared to conventional methods.

Keywords:
(100.0100) Image processing(110.0110) Imaging systems(110.4500) Optical coherence tomography(170.0170) Medical optics and biotechnology(170.0180) Microscopy(170.3890) Medical optics instrumentation(180.6900) Three-dimensional microscopy

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

12.0K
Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.3K

Related Experiment Videos

Last Updated: Mar 6, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

9.0K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

12.0K
Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.3K

Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Coherent Tomography

Background:

  • Conventional swept source optical coherence tomography (SS-OCT) systems typically use Fast Fourier Transform (FFT) for image reconstruction.
  • Existing methods may struggle with dynamic focusing and dispersion compensation.
  • There is a need for improved OCT techniques offering higher resolution and faster data processing.

Purpose of the Study:

  • To apply the Gabor filtering protocol to a Master/Slave (MS) swept source optical coherence tomography (SS-OCT) system operating at 1300 nm.
  • To demonstrate the advantages of the MS principle for depth-specific signal acquisition and dynamic focusing.
  • To showcase the combined benefits of Gabor filtering and MS-OCT for imaging and data processing.

Main Methods:

  • Implementation of Gabor filtering on a 1300 nm Master/Slave (MS) swept source optical coherence tomography (SS-OCT) system.
  • Acquisition of multiple data sets from different focus positions to construct volumetric data.
  • Utilizing the MS principle for depth-specific signal extraction and automatic dispersion compensation.

Main Results:

  • Successful application of Gabor filtering to MS-SS-OCT, enabling dynamic focusing and depth-specific signal retrieval.
  • Generation of volumetric data with constant transversal resolution from biological samples (cucumber) and insect specimens.
  • Demonstration of simultaneous display of multiple image types (en-face, cross-sectional, confocal-like) within a single frame.
  • MS-OCT combined with Gabor filtering showed superior speed in assembling fused volumes compared to FFT-based SS-OCT when more than 4 focus positions were needed.

Conclusions:

  • The combination of Gabor filtering and MS-OCT provides a powerful imaging instrument with enhanced capabilities.
  • The MS principle offers advantages in dynamic focusing, depth-specific signal delivery, and dispersion tolerance.
  • This integrated approach demonstrates improved speed and efficiency for volumetric OCT imaging, particularly in complex scenarios.