Influence of water content on Raman spectroscopy characterization of skin sample

We report that the Raman spectrum obtained from porcine skin varies significantly with the change of skin water content. At different water contents from 40 to 55 wt.%, the Raman spectra results using confocal Raman spectroscopy show that the spectral variation of porcine skin is highly affected by skin water content. Experimental data are consistent with the Monte Carlo calculation and it is proved that the intensity of the Raman spectrum depends on the angle distribution and collection efficiency of backscattered light from the sample surface for a varied water content. It is suggested that water content for a given skin sample should be controlled carefully to minimize errors and deviations in the Raman peak analyses.