Establishment of Experimental Murine Peritonitis Model with Hog Gastric Mucin for Carbapenem-Resistant Gram-Negative...

Area of Science:

• Infectious disease research
• Microbiology
• Animal modeling

Background:

• Animal models are crucial for studying infectious diseases, with mice commonly used for bacterial infection research.
• Existing methods require adaptation for clinical isolates, especially carbapenem-resistant bacteria, depending on the specific infection model and pathogen.
• Tailored approaches are necessary for accurate preclinical evaluation of antimicrobial efficacy.

Purpose of the Study:

• To develop and validate an efficient and applicable animal model for investigating carbapenem-resistant Gram-negative bacterial infections.
• To assess the utility of a specific infection method using BALB/c mice and hog gastric mucin.
• To provide a reliable model for studying prevalent carbapenem-resistant pathogens in Korea.

Main Methods:

• Infection of 6-week-old female BALB/c mice via intraperitoneal injection.
• Administration of various strains of carbapenem-resistant bacteria.
• Inclusion of 3% hog gastric mucin to enhance infection establishment.

Main Results:

• The developed method proved efficient for infecting mice with carbapenem-resistant Gram-negative bacteria.
• The model was readily applicable to key pathogens including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii.
• Successful establishment of infection in the murine model was achieved.

Conclusions:

• The intraperitoneal infection model using hog gastric mucin is effective for studying carbapenem-resistant Gram-negative pathogens.
• This model is suitable for research on prevalent multidrug-resistant bacteria in Korea.
• The method offers a valuable tool for preclinical assessment of novel therapeutics against resistant infections.