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Related Concept Videos

Introduction to Nuclear Reprogramming01:14

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
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Engineering Cell-permeable Protein
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Cellular Reprogramming Using Protein and Cell-Penetrating Peptides.

Bong Jong Seo1, Yean Ju Hong2, Jeong Tae Do3

  • 1Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea. sbj1990@naver.com.

International Journal of Molecular Sciences
|March 10, 2017
PubMed
Summary

Induced pluripotent stem cells (iPSCs) offer promising cell therapy potential due to their regenerative capabilities. This study details current iPSC generation technologies, focusing on efficient protein-based transduction methods.

Keywords:
cell penetrating peptideiPSCsproteinreprogramming

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Area of Science:

  • Biotechnology and Regenerative Medicine
  • Stem Cell Biology

Background:

  • Stem cells are crucial for cell therapy due to self-renewal and differentiation potential.
  • Ethical and efficiency considerations favor induced pluripotent stem cells (iPSCs) for therapeutic applications.

Purpose of the Study:

  • To provide an overview of current induced pluripotent stem cell (iPSC) generation technologies.
  • To detail protein-based transduction as a method for generating iPSCs.

Main Methods:

  • Review of existing stem cell generation techniques, including nuclear transfer and direct reprogramming.
  • Focus on various transduction methods for creating induced pluripotent stem cells (iPSCs): viral, plasmid, RNA, and protein-based.
  • Detailed explanation of protein-based transduction technology for iPSC generation.

Main Results:

  • Induced pluripotent stem cells (iPSCs) are a highly promising stem cell source for cell therapy.
  • Protein-based transduction is presented as a key technology for efficient iPSC generation.

Conclusions:

  • Induced pluripotent stem cells (iPSCs) represent a significant advancement in cell therapy.
  • Protein-based transduction offers a viable and detailed method for their generation, overcoming some limitations of other techniques.