Lysozyme distribution, structural identification, and in vitro release of starch-based microgel-lysozyme complexes

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Gastroenterology Research

Background:

• Lysozyme is a crucial enzyme with therapeutic potential.
• Protecting enzymes like lysozyme from degradation in the gastrointestinal tract is challenging.
• Developing effective delivery systems for targeted release is essential for maximizing therapeutic efficacy.

Purpose of the Study:

• To investigate the formation and distribution of microgel-lysozyme complexes.
• To evaluate the structural integrity of lysozyme within the microgel carrier.
• To assess the in vitro release profile and gastrointestinal stability of lysozyme from the microgel system.

Main Methods:

• Confocal laser scanning microscopy (CLSM) for visualizing complex formation and distribution.
• Fourier transform infrared (FT-IR) spectroscopy and Raman spectroscopy for structural analysis of lysozyme.
• In vitro release studies simulating gastrointestinal conditions.
• Circular dichroism (CD) spectroscopy to evaluate lysozyme structure after release.

Main Results:

• CLSM confirmed homogeneous distribution of lysozyme within the microgel particles.
• FT-IR and Raman spectra indicated that lysozyme's secondary structure remained intact.
• In vitro release studies demonstrated protection against premature release in simulated stomach conditions.
• CD analysis confirmed lysozyme structural integrity after simulated gastric exposure.

Conclusions:

• Carboxymethyl starch-based microgels serve as a viable carrier system for lysozyme.
• The microgels effectively protect lysozyme from degradation in the stomach.
• This system shows potential for targeted delivery of lysozyme to the intestine for gastrointestinal applications.