Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

1.2K
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
1.2K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

872
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
872
Bacterial Signaling01:30

Bacterial Signaling

42.4K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
42.4K
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

807
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
807
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

10.5K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
10.5K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

112.5K
Overview
112.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SHY2 as a node in the regulation of root meristem development by auxin, brassinosteroids, and cytokinin.

Journal of integrative plant biology·2020
Same author

Dynamic tailoring of an optical skyrmion lattice in surface plasmon polaritons.

Optics express·2020
Same author

High-Efficiency, Broadband, Near Diffraction-Limited, Dielectric Metalens in Ultraviolet Spectrum.

Nanomaterials (Basel, Switzerland)·2020
Same author

Experimental Examination of Electrical Characteristics for Portland Cement Mortar Frost Damage Evaluation.

Materials (Basel, Switzerland)·2020
Same author

Synthesis of Imidazole-Based Medicinal Molecules Utilizing the van Leusen Imidazole Synthesis.

Pharmaceuticals (Basel, Switzerland)·2020
Same author

Mutagenesis for Improvement of Activity and Stability of Prolyl Aminopeptidase from Aspergillus oryzae.

Applied biochemistry and biotechnology·2020

Related Experiment Video

Updated: Mar 6, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.7K

Multimer recognition and secretion by the non-classical secretion pathway in Bacillus subtilis.

Liuqun Zhao1,2, Jingqi Chen1,2, Jibin Sun1,2

  • 1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, P. R. China.

Scientific Reports
|March 10, 2017
PubMed
Summary

Folded multimeric proteins are secreted by a non-classical pathway in Bacillus subtilis. This pathway recognizes intact tetrameric protein complexes, suggesting a multimer-specific secretion mechanism.

More Related Videos

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
08:58

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Published on: April 15, 2019

10.7K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

21.2K

Related Experiment Videos

Last Updated: Mar 6, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.7K
Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
08:58

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Published on: April 15, 2019

10.7K
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

21.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Protein Secretion

Background:

  • Non-classical protein secretion is common in bacteria, but the underlying principles are not fully understood.
  • Existing knowledge does not clarify the selection criteria for proteins entering these pathways.

Purpose of the Study:

  • To investigate the secretion mechanism of the cytoplasmic protein D-psicose 3-epimerase (RDPE) in Bacillus subtilis.
  • To determine if folded multimeric proteins can be secreted via non-classical pathways.
  • To elucidate the structural requirements and recognition principles for non-classical protein secretion.

Main Methods:

  • Analysis of truncation variants and alanine scanning mutagenesis of RDPE.
  • Construction of reporter fusions (mCherry, GFP) to study secretion regions.
  • Use of cross-linked tetramers to assess secretion of intact complexes.
  • Microscopic examination of protein localization within bacterial cells.

Main Results:

  • Non-classical secretion of RDPE is independent of cell lysis and requires specific structural features, including C- and N-termini and hydrophobic domains.
  • Hydrophobic residues are critical for maintaining the folded/unfolded equilibrium necessary for secretion.
  • Secretion is dependent on an intact tetrameric complex; folded tetramers are secreted as a single unit.
  • The non-classical secretion pathway exhibits a preference for multimeric substrates, which localize to cell poles and the septum.

Conclusions:

  • Folded multimeric proteins can be secreted via a non-classical pathway in Bacillus subtilis.
  • A multimer recognition mechanism is likely central to this non-classical secretion pathway.
  • Structural integrity and quaternary structure are key determinants for secretion via this route.