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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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A colorimetric nanosensor based on a selective target-responsive aptamer kissing complex.

E Goux1, E Dausse2, V Guieu1

  • 1University Grenoble Alpes, DPM CNRS UMR 5063, F-38041 Grenoble, France. eric.peyrin@univ-grenoble-alpes.fr valerie.guieu@univ-grenoble-alpes.fr.

Nanoscale
|March 10, 2017
PubMed
Summary

We developed a novel colorimetric aptasensor using gold nanoparticles. This sensor detects analytes via DNA and RNA hairpin interactions, causing a visible color change.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Colorimetric aptasensors offer sensitive detection methods.
  • Gold nanoparticles are widely used due to their unique optical properties.
  • Analyte detection often requires complex signal transduction.

Purpose of the Study:

  • To develop a novel colorimetric aptasensor.
  • To utilize functionalized gold nanoparticle probes for analyte detection.
  • To demonstrate a new mechanism for signal generation based on hairpin interactions.

Main Methods:

  • Conjugation of gold nanoparticles with two functional DNA and RNA hairpins.
  • Utilizing kissing (loop-loop) interactions between hairpins for analyte binding.
  • Observing color change from red to purple due to nanoparticle aggregation.

Main Results:

  • Successful design of a colorimetric aptasensor.
  • Demonstration of analyte-induced hairpin interactions.
  • Visible color change indicating successful detection and aggregation.

Conclusions:

  • The developed aptasensor provides a novel colorimetric detection method.
  • Kissing interactions of DNA/RNA hairpins offer a viable mechanism for aptasensor signal transduction.
  • This approach enables sensitive detection of small analyte ligands.