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Updated: Mar 6, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Metabolic divergence in simultaneous biological removal of nitrate and sulfide for elemental sulfur production under
Michal Sposob1, Rune Bakke1, Carlos Dinamarca1
1University College of Southeast Norway, Kjølnes Ring 56, Porsgrunn 3918, Norway.
Abstract:
The simultaneous removal of NO3- and HS- at temperature stress (25-10°C) is evaluated here. An expanded granular sludge bed (EGSB) reactor was run over 120days at N/S molar ratio of 0.35 (for S0 production) under constant sulfur loading rate of 0.4kgS/m3d. The simultaneous removal of NO3- and HS-, was achieved at applied conditions. Average HS--S removal varied from 98 (25°C) to 89.2% at 10°C, with almost complete NO3- removal. Average S0 yield ranged from 83.7 at 25°C to 67% at 10°C. The temperature drop caused a decrease in granular sludge accumulated S0 fraction by nearly 2.5 times. Decreased temperature caused metabolic pathway change observed as higher SO42- production, apparently allowing the biomass to obtain more energy per HS- consumed. It is hypothesized that the metabolic shift is a natural response to compensate for temperature-induced changes in energy requirements.
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