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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
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Two-Dimensional Surface Plasmon Resonance Imaging System for Cellular Analysis
Tanveer Ahmad Mir1,2,3,4, Hiroaki Shinohara5,6
1Graduate School of Science and Engineering for Research, University of Toyama, Gofuku 3190, Toyama-shi, Toyama, 930-8555, Japan. tanveer9@eng.u-toyama.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2017
Summary
High-resolution surface plasmon resonance imaging (SPRi) enables real-time monitoring of individual cell responses. This optical biosensor technology successfully detected neuronal differentiation and protein kinase C translocation in live cells.
Area of Science:
- Biotechnology
- Cell Biology
- Biosensing
Background:
- Surface plasmon resonance (SPR) and SPR imaging (SPRi) are valuable optical biosensor techniques for cellular analysis.
- Existing methods often average cell responses, masking crucial individual cell-level molecular interactions.
- High-resolution SPRi is needed to fully evaluate compound effects on complex cellular systems.
Purpose of the Study:
- To explore the utility of high-resolution 2D-SPR imaging for real-time monitoring of intracellular protein kinase C (PKC) translocation.
- To detect neuronal differentiation in live cells at the single-cell level using 2D-SPR imaging.
- To investigate the response of differentiated PC12 cells to muscarine stimulation.
Main Methods:
- Utilized a high-resolution 2D-SPR imaging system for cellular analysis.
- Employed the PC12 cell line, a common model for neuronal differentiation research.
- Applied nerve growth factor (NGF) to induce neuronal differentiation in PC12 cells.
Main Results:
- Successfully generated 2D-SPR signals and images from live cells.
- Observed enhanced SPR response in NGF-treated cells upon stimulation with muscarine.
- Demonstrated the capability of 2D-SPR imaging to monitor intracellular events and cellular differentiation.
Conclusions:
- High-resolution 2D-SPR imaging is effective for real-time, single-cell level monitoring of cellular processes.
- This technique can detect intracellular protein translocation and neuronal differentiation.
- SPRi offers a powerful tool for evaluating drug potency, specificity, and toxicity at the individual cell level.
