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Two-Dimensional Surface Plasmon Resonance Imaging System for Cellular Analysis.

Tanveer Ahmad Mir1,2,3,4, Hiroaki Shinohara5,6

  • 1Graduate School of Science and Engineering for Research, University of Toyama, Gofuku 3190, Toyama-shi, Toyama, 930-8555, Japan. tanveer9@eng.u-toyama.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2017
PubMed
Summary
This summary is machine-generated.

High-resolution surface plasmon resonance imaging (SPRi) enables real-time monitoring of individual cell responses. This optical biosensor technology successfully detected neuronal differentiation and protein kinase C translocation in live cells.

Keywords:
MuscarineNerve growth factorProtein kinase CSurface plasmon resonanceSurface plasmon resonance imaging

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Biosensing

Background:

  • Surface plasmon resonance (SPR) and SPR imaging (SPRi) are valuable optical biosensor techniques for cellular analysis.
  • Existing methods often average cell responses, masking crucial individual cell-level molecular interactions.
  • High-resolution SPRi is needed to fully evaluate compound effects on complex cellular systems.

Purpose of the Study:

  • To explore the utility of high-resolution 2D-SPR imaging for real-time monitoring of intracellular protein kinase C (PKC) translocation.
  • To detect neuronal differentiation in live cells at the single-cell level using 2D-SPR imaging.
  • To investigate the response of differentiated PC12 cells to muscarine stimulation.

Main Methods:

  • Utilized a high-resolution 2D-SPR imaging system for cellular analysis.
  • Employed the PC12 cell line, a common model for neuronal differentiation research.
  • Applied nerve growth factor (NGF) to induce neuronal differentiation in PC12 cells.

Main Results:

  • Successfully generated 2D-SPR signals and images from live cells.
  • Observed enhanced SPR response in NGF-treated cells upon stimulation with muscarine.
  • Demonstrated the capability of 2D-SPR imaging to monitor intracellular events and cellular differentiation.

Conclusions:

  • High-resolution 2D-SPR imaging is effective for real-time, single-cell level monitoring of cellular processes.
  • This technique can detect intracellular protein translocation and neuronal differentiation.
  • SPRi offers a powerful tool for evaluating drug potency, specificity, and toxicity at the individual cell level.