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Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
Changwon Lee1, Peng Wang1, Marsha A Gaston2
1Department of Chemistry, University of Cincinnati, Cincinnati, OH, 45221, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2017
Summary
A novel biosensor detects viruses using gold nanoparticles and their color change upon aggregation. This method, based on hemagglutinin-sialic acid interactions, offers sensitive influenza B virus detection.
Area of Science:
- Nanotechnology
- Biotechnology
- Biosensing
Background:
- Viral detection is crucial for public health.
- Existing methods can be complex and time-consuming.
- Gold nanoparticles offer unique optical properties for sensing applications.
Purpose of the Study:
- To develop a novel, sensitive, and rapid biosensor for virus detection.
- To utilize the plasmonic properties of gold nanoparticles for viral detection.
- To leverage the specific interaction between viral hemagglutinin and sialic acid.
Main Methods:
- Synthesized sialic acid-stabilized gold nanoparticles using a green chemistry approach.
- Utilized the plasmonic peak shift phenomenon of gold nanoparticles upon aggregation.
- Induce nanoparticle aggregation via hemagglutinin-sialic acid binding with viral particles.
- Measured changes in optical density to quantify virus concentration.
Main Results:
- Achieved target-specific aggregation of gold nanoparticles with viral particles.
- Observed a linear correlation between optical density changes and influenza B virus dilution.
- Established a detection limit of 0.156 vol% for virus dilution (hemagglutination assay titer, 512).
Conclusions:
- The developed gold nanoparticle-based biosensor effectively detects influenza B virus.
- The biosensor utilizes a simple, green synthesis method and relies on specific biomolecular interactions.
- The system demonstrates potential for sensitive and rapid viral detection, with linearity extendable by increasing sialic acid-gold nanoparticle concentration.
