Reflectometric Interference Spectroscopy
Guenther Proll1,2, Goran Markovic3, Peter Fechner3
1Institute of Physical and Theoretical Chemistry, University of Tuebingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany. guenther.proll@ipc.uni-tuebingen.de.
Abstract:
Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance. The advantages of direct optical detection depend on a sophisticated surface chemistry resulting negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.
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