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Related Experiment Videos

Structure of cryptic lambda prophages.

R J Redfield1, A M Campbell

  • 1Department of Biological Sciences, Stanford University, CA 94305.

Journal of Molecular Biology
|December 5, 1987
PubMed
Summary
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Ultraviolet light induction of Escherichia coli with bacteriophage lambda can create cryptic lysogens. These variants feature large, stable chromosomal duplications, potentially impacting bacterial evolution.

Area of Science:

  • Microbiology
  • Bacteriology
  • Genetics

Background:

  • Bacteriophage lambda lysogeny in Escherichia coli can result in cryptic prophages upon induction.
  • A specific lambda variant, lambda crg, containing an IS2 insertion, significantly increases cryptic lysogen frequency.

Purpose of the Study:

  • To characterize the genetic makeup of cryptic lysogens formed after lambda induction.
  • To understand the mechanisms generating large chromosomal duplications in these lysogens.

Main Methods:

  • Induction of lambda lysogenic Escherichia coli with ultraviolet light.
  • Characterization of 43 cryptic prophages from lambda crg and 5 from a reference lambda strain.
  • Analysis of recombination events and duplicated host DNA segments.

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Main Results:

  • Cryptic prophages often contain large substitutions replacing early phage genome segments with host chromosomal DNA.
  • Recombination events involving IS2 elements and the Qsr' prophage generate these duplications, some exceeding 200,000 bases.
  • These large duplications are stable and distinct from tandem duplications.

Conclusions:

  • The IS2 insertion in lambda crg facilitates the formation of stable, large host DNA duplications.
  • These stable duplications may confer a selective advantage, influencing bacterial evolution.
  • Mechanisms involve specific recombination events within the prophage and host genomes.